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anti dr4  (ProSci Incorporated)


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    Structured Review

    ProSci Incorporated anti dr4
    Anti Dr4, supplied by ProSci Incorporated, used in various techniques. Bioz Stars score: 94/100, based on 32 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    Related Articles

    Western Blot:

    Article Title: The Identification of New c-FLIP Inhibitors for Restoring Apoptosis in TRAIL-Resistant Cancer Cells.
    Article Snippet: The catalytically inactive caspase-8-homologous protein, c-FLIP, is a potent antiapoptotic protein highly expressed in various types of cancers. c-FLIP competes with caspase-8 for binding to the adaptor protein FADD (Fas-Associated Death Domain) following death receptors’ (DRs) activation via the ligands of the TNF-R family.. As a consequence, the extrinsic apoptotic signaling pathway involving DRs is inhibited.. The inhibition of c-FLIP activity in tumor cells might enhance DR-mediated apoptosis and overcome immune and anticancer drug resistance.

    Article Title: The Identification of New c-FLIP Inhibitors for Restoring Apoptosis in TRAIL-Resistant Cancer Cells
    Article Snippet: For Western blotting (WB) experiments, we used the following antibodies: anti-FLIP antibodies DAVE2 and NF6 (Adipogen, San Diego, CA, USA), caspase-3 (8G10; OZYME), PARP (Asp214, 19F4; OZYME, Saint-Cyr-l’École, France), caspase-8 (1C12; OZYME), anti-MBP (New England Biolabs, Ipswich, MA, USA) and anti-His (C-ter) (Invitrogen, Carlsbad, CA, USA). .. Anti-His (ab81663) was used to immunoprecipitate the DISC complex, and the following antibodies were used for WB analysis: anti-Flip (DAVE II, Adipogen), anti-FADD (556402, BD, San Jose CA, USA), anti-casp8 (5F7, EnzoLife, Farmingdale, NY, USA), anti-DR4 (1139, ProSci, Poway, CA, USA) and anti-DR5 (3696, Cell Signaling, Danvers, MA, USA). .. Anti-rabbit and anti-mouse HRP-linked secondary antibodies (Santa Cruz Biotechnology, Dallas, TX, USA) and β-actin (Sigma Aldrich, St. Louis, MO, USA) were also used.

    Article Title: Compositions and methods related to tumor cell killers and vaccines
    Article Snippet: .. Antibodies for Western blot and flow cytometry analysis: Antibodies against p-44/42MAPK (ERK1/2) (Cell Signaling), cleaved PARP (Cell Signaling), caspase 8 (Cell Signaling), α-tubulin (Sigma), anti-FLAG (Sigma), anti-DR4 (ProSci), anti-DR5 (ProSci) and anti-TRAIL (Abcam) were used for western blotting. .. Anti-human CD261 (DR4) PE (eBioscience), and anti-human CD262 (DR5) PE (eBioscience) were used for flow cytometry analysis.

    Recombinant:

    Article Title: Anti-EGFR VHH-armed death receptor ligand–engineered allogeneic stem cells have therapeutic efficacy in diverse brain metastatic breast cancers
    Article Snippet: .. Antibodies against β-actin (#4970), phospho-AKT (Ser 473 , #4060), AKT (#9272), caspase-7 (#9492), caspase-8 (#9746), caspase-9 (#9508), cleaved caspase-3 (#9661), EGFR (#2646 and #4267), phospho-EGFR (Tyr 1068 , #3777), cleaved poly(ADP-ribose) polymerase (PARP; #9541), phospho-p44/42 mitogen-activated protein kinase (MAPK) (ERK1/2) (Thr 202 /Tyr 204 , #9101), p-44/42 MAPK (ERK1/2) (#9102), Fas-associated death domain protein (#2782), Bcl-2 (#2872), Bcl-xL (#2764), XIAP (#2042), cIAP2 (#3130), phospho–signal transducers and activators of transcription 3 (STAT3) (Tyr 705 , #9145), STAT3 (#4904), HER2 (#2242), horseradish peroxidase (HRP) anti-rabbit (#7074), Rab5 (#46449), Rab7 (#95746) (Cell Signaling Technology), anti–nuclear factor κB (#ab16502), anti-TRAIL (#ab9959), anti-CD31 (#ab28364), HRP anti-mouse (#ab205719) (Abcam), anti–α-tubulin (#T5168), anti-Vinculin (#V4505), NeuN (#MAB377), glial fibrillary acidic protein (GFAP) (#MAB3402) (Sigma-Aldrich), Alexa Fluor 488 anti-EGFR antibody (#352908), anti-DR4 (#1139), anti-DR5 (#2019) (ProSci), anti-DR4 (#sc-32255), anti-DR5 (#sc-166624), anti-cIAP1 (#sc-271419), normal mouse IgG (#sc-2025) (Santa Cruz), anti-Ki-67 (#180191Z), anti-GFAP (#180063), Alexa Fluor anti-rabbit 405 (#A-31556), Alexa Fluor anti-rabbit 488 (#A-11008), Alexa Fluor anti-mouse 555 (#A-21422), Alexa Flour anti-rabbit 647 (#A-21244), Phycoerythrin (PE) anti-DR4 (#12-6644-42), PE anti-DR5 (#12-9908-42), PE mouse IgG isotype (#12-4714-42) (Invitrogen), Cetuximab (ImClone Systems), Erlotinib (#SYN-1039, Selleck Chemicals), human recombinant EGF (R&D Systems), PE anti-EGFR (#352903, BioLegend), and IBA1 (#019-19741, FUJIFILM). .. TNBC patient tissue samples were obtained from Massachusetts General Hospital as approved by institutional review board (IRB) at Harvard Medical School, Keio University Hospital as approved by IRB of Keio University School of Medicine, and US Biomax Inc. (#BR1901).

    Flow Cytometry:

    Article Title: Compositions and methods related to tumor cell killers and vaccines
    Article Snippet: .. Antibodies for Western blot and flow cytometry analysis: Antibodies against p-44/42MAPK (ERK1/2) (Cell Signaling), cleaved PARP (Cell Signaling), caspase 8 (Cell Signaling), α-tubulin (Sigma), anti-FLAG (Sigma), anti-DR4 (ProSci), anti-DR5 (ProSci) and anti-TRAIL (Abcam) were used for western blotting. .. Anti-human CD261 (DR4) PE (eBioscience), and anti-human CD262 (DR5) PE (eBioscience) were used for flow cytometry analysis.

    Transferring:

    Article Title: Lipocalin 2 inversely regulates TRAIL sensitivity through p38 MAPK-mediated DR5 regulation in colorectal cancer.
    Article Snippet: .. After transferring and blocking using 3% of bovine albumin serum, the polyvinylidene difluoride (PVDF) membranes were probed with various antibodies [anti-LCN2 (1:1,000; AF1757; R&D Systems,Minneapolis, MN, USA), anti-DR4 (1:1,000; #1167; ProSci ΨTM, Poway, CA, USA), anti-DR5 (1:1,000; #2019; ProSci ΨTM), anti-Fasassociated death-domain-like IL-1β-converting enzyme (FLICE)-inhibitory protein (FLIP, 1:1,000; #8510; Cell Signaling Technology, Danvers, MA, USA), anti-Bid (1:1,000; SC-11423; Santa Cruz Biotechnology), anti-caspase-8 (1:1,000; SC-73526; Santa Cruz Biotechnology), anti-caspase-3 (1:1,000; SC-7148; Santa Cruz Biotechnology), anti-cleaved caspase-3 (1:1,000; #9661; Cell Signaling Technology), anti-poly(ADP-ribose) polymerase (PARP, 1:1,000, SC-7150; Santa Cruz Biotechnology), anti-Bcl-xL (1:1,000; SC-8392; Santa Cruz Biotechnology), anti-Bcl-2 (1:1,000; SC-783; Santa Cruz Biotechnology), anti-cytochrome c (1:1,000, SC-65396; Santa Cruz Biotechnology), anti-caspase-9 (1:1,000; #9502S; Cell Signaling Technology), anti-phospho-ERK (1:1,000; #9106; Cell Signaling Technology), anti-phospho-p38 (1:1,000, #4511; Cell Signaling Technology), anti-phosphoJNK (1:1,000; #4668; Cell Signaling Technology), anti-C/EBP homologous protein (CHOP, 1:1,000; #2895; Cell Signaling Technology) and anti-actin (1:2,000; A2066; Sigma-Aldrich) antibodies] in 4 ̊C for overnight. .. HRP-conjugated goat anti-rabbit IgG (SC-2004; Santa Cruz Biotechnology), goat anti-mouse (SC-2005; Santa Cruz Biotechnology) and mouse anti-goat (SC-2354; Santa Cruz Biotechnology) secondary antibodies were used at a concentration of 1:3,000 for 1 h at room temperature.

    Blocking Assay:

    Article Title: Lipocalin 2 inversely regulates TRAIL sensitivity through p38 MAPK-mediated DR5 regulation in colorectal cancer.
    Article Snippet: .. After transferring and blocking using 3% of bovine albumin serum, the polyvinylidene difluoride (PVDF) membranes were probed with various antibodies [anti-LCN2 (1:1,000; AF1757; R&D Systems,Minneapolis, MN, USA), anti-DR4 (1:1,000; #1167; ProSci ΨTM, Poway, CA, USA), anti-DR5 (1:1,000; #2019; ProSci ΨTM), anti-Fasassociated death-domain-like IL-1β-converting enzyme (FLICE)-inhibitory protein (FLIP, 1:1,000; #8510; Cell Signaling Technology, Danvers, MA, USA), anti-Bid (1:1,000; SC-11423; Santa Cruz Biotechnology), anti-caspase-8 (1:1,000; SC-73526; Santa Cruz Biotechnology), anti-caspase-3 (1:1,000; SC-7148; Santa Cruz Biotechnology), anti-cleaved caspase-3 (1:1,000; #9661; Cell Signaling Technology), anti-poly(ADP-ribose) polymerase (PARP, 1:1,000, SC-7150; Santa Cruz Biotechnology), anti-Bcl-xL (1:1,000; SC-8392; Santa Cruz Biotechnology), anti-Bcl-2 (1:1,000; SC-783; Santa Cruz Biotechnology), anti-cytochrome c (1:1,000, SC-65396; Santa Cruz Biotechnology), anti-caspase-9 (1:1,000; #9502S; Cell Signaling Technology), anti-phospho-ERK (1:1,000; #9106; Cell Signaling Technology), anti-phospho-p38 (1:1,000, #4511; Cell Signaling Technology), anti-phosphoJNK (1:1,000; #4668; Cell Signaling Technology), anti-C/EBP homologous protein (CHOP, 1:1,000; #2895; Cell Signaling Technology) and anti-actin (1:2,000; A2066; Sigma-Aldrich) antibodies] in 4 ̊C for overnight. .. HRP-conjugated goat anti-rabbit IgG (SC-2004; Santa Cruz Biotechnology), goat anti-mouse (SC-2005; Santa Cruz Biotechnology) and mouse anti-goat (SC-2354; Santa Cruz Biotechnology) secondary antibodies were used at a concentration of 1:3,000 for 1 h at room temperature.



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    dDNMT activates TRAIL-death receptor signaling in VHL -deficient ccRCC cells (A) Volcano plot of SGI1027-induced and -repressed genes in RCC10 cells ( n = 2 biological replicates). FC, fold change. (B) Biocarta pathway enrichment analysis of SGI1027-induced genes in RCC10 cells. (C) RT-qPCR analysis of TNFSF10 , TNFRSF10A , TNFRSF10B , and TNFRSF10D mRNA levels in RCC10 cells treated with vehicle or SGI1027 for 2 days ( n = 3 biological replicates). (D and E) Immunoblot analysis of TRAIL, <t>DR4,</t> DR5, DcR2, pro-caspase-10, and cleaved caspase-10 (C-caspase-10) proteins in isogenic RCC10 cells treated with vehicle, SGI1027 (D and E, n = 2 biological replicates), MS1129 (E, n = 2 biological replicates), or decitabine (E, n = 2 biological replicates) for 2 or 7 days. (F) Global m5C levels in RCC10 cells treated with vehicle or SGI1027 for 2 days by ELISA assay ( n = 3 biological replicates). (G–J) MeDIP-qPCR assay in RCC10 cells treated with vehicle or SGI1027 for 2 days ( n = 3 biological replicates). (K–M) DNMT1, DNMT3A, and DNMT3B ChIP-qPCR assay in RCC10 cells ( n = 3 biological replicates). (N) Scheme of dDNMT-activated apoptotic pathway. Data represent mean ± SEM. p value was determined by bioinformatics with edgeR (A) or gene set enrichment analysis (B), unpaired 2-tailed Student’s t test (C and F), two-way ANOVA with Tukey’s test (G–I), and one-way ANOVA with Dunnett’s test (K–M). See also and .
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    dDNMT activates TRAIL-death receptor signaling in VHL -deficient ccRCC cells (A) Volcano plot of SGI1027-induced and -repressed genes in RCC10 cells ( n = 2 biological replicates). FC, fold change. (B) Biocarta pathway enrichment analysis of SGI1027-induced genes in RCC10 cells. (C) RT-qPCR analysis of TNFSF10 , TNFRSF10A , TNFRSF10B , and TNFRSF10D mRNA levels in RCC10 cells treated with vehicle or SGI1027 for 2 days ( n = 3 biological replicates). (D and E) Immunoblot analysis of TRAIL, <t>DR4,</t> DR5, DcR2, pro-caspase-10, and cleaved caspase-10 (C-caspase-10) proteins in isogenic RCC10 cells treated with vehicle, SGI1027 (D and E, n = 2 biological replicates), MS1129 (E, n = 2 biological replicates), or decitabine (E, n = 2 biological replicates) for 2 or 7 days. (F) Global m5C levels in RCC10 cells treated with vehicle or SGI1027 for 2 days by ELISA assay ( n = 3 biological replicates). (G–J) MeDIP-qPCR assay in RCC10 cells treated with vehicle or SGI1027 for 2 days ( n = 3 biological replicates). (K–M) DNMT1, DNMT3A, and DNMT3B ChIP-qPCR assay in RCC10 cells ( n = 3 biological replicates). (N) Scheme of dDNMT-activated apoptotic pathway. Data represent mean ± SEM. p value was determined by bioinformatics with edgeR (A) or gene set enrichment analysis (B), unpaired 2-tailed Student’s t test (C and F), two-way ANOVA with Tukey’s test (G–I), and one-way ANOVA with Dunnett’s test (K–M). See also and .
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    dDNMT activates TRAIL-death receptor signaling in VHL -deficient ccRCC cells (A) Volcano plot of SGI1027-induced and -repressed genes in RCC10 cells ( n = 2 biological replicates). FC, fold change. (B) Biocarta pathway enrichment analysis of SGI1027-induced genes in RCC10 cells. (C) RT-qPCR analysis of TNFSF10 , TNFRSF10A , TNFRSF10B , and TNFRSF10D mRNA levels in RCC10 cells treated with vehicle or SGI1027 for 2 days ( n = 3 biological replicates). (D and E) Immunoblot analysis of TRAIL, <t>DR4,</t> DR5, DcR2, pro-caspase-10, and cleaved caspase-10 (C-caspase-10) proteins in isogenic RCC10 cells treated with vehicle, SGI1027 (D and E, n = 2 biological replicates), MS1129 (E, n = 2 biological replicates), or decitabine (E, n = 2 biological replicates) for 2 or 7 days. (F) Global m5C levels in RCC10 cells treated with vehicle or SGI1027 for 2 days by ELISA assay ( n = 3 biological replicates). (G–J) MeDIP-qPCR assay in RCC10 cells treated with vehicle or SGI1027 for 2 days ( n = 3 biological replicates). (K–M) DNMT1, DNMT3A, and DNMT3B ChIP-qPCR assay in RCC10 cells ( n = 3 biological replicates). (N) Scheme of dDNMT-activated apoptotic pathway. Data represent mean ± SEM. p value was determined by bioinformatics with edgeR (A) or gene set enrichment analysis (B), unpaired 2-tailed Student’s t test (C and F), two-way ANOVA with Tukey’s test (G–I), and one-way ANOVA with Dunnett’s test (K–M). See also and .
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    Image Search Results


    dDNMT activates TRAIL-death receptor signaling in VHL -deficient ccRCC cells (A) Volcano plot of SGI1027-induced and -repressed genes in RCC10 cells ( n = 2 biological replicates). FC, fold change. (B) Biocarta pathway enrichment analysis of SGI1027-induced genes in RCC10 cells. (C) RT-qPCR analysis of TNFSF10 , TNFRSF10A , TNFRSF10B , and TNFRSF10D mRNA levels in RCC10 cells treated with vehicle or SGI1027 for 2 days ( n = 3 biological replicates). (D and E) Immunoblot analysis of TRAIL, DR4, DR5, DcR2, pro-caspase-10, and cleaved caspase-10 (C-caspase-10) proteins in isogenic RCC10 cells treated with vehicle, SGI1027 (D and E, n = 2 biological replicates), MS1129 (E, n = 2 biological replicates), or decitabine (E, n = 2 biological replicates) for 2 or 7 days. (F) Global m5C levels in RCC10 cells treated with vehicle or SGI1027 for 2 days by ELISA assay ( n = 3 biological replicates). (G–J) MeDIP-qPCR assay in RCC10 cells treated with vehicle or SGI1027 for 2 days ( n = 3 biological replicates). (K–M) DNMT1, DNMT3A, and DNMT3B ChIP-qPCR assay in RCC10 cells ( n = 3 biological replicates). (N) Scheme of dDNMT-activated apoptotic pathway. Data represent mean ± SEM. p value was determined by bioinformatics with edgeR (A) or gene set enrichment analysis (B), unpaired 2-tailed Student’s t test (C and F), two-way ANOVA with Tukey’s test (G–I), and one-way ANOVA with Dunnett’s test (K–M). See also and .

    Journal: Cell Reports Medicine

    Article Title: HIF-activated priming of TRAIL-induced cell death determines epigenetic vulnerability in kidney cancer

    doi: 10.1016/j.xcrm.2026.102630

    Figure Lengend Snippet: dDNMT activates TRAIL-death receptor signaling in VHL -deficient ccRCC cells (A) Volcano plot of SGI1027-induced and -repressed genes in RCC10 cells ( n = 2 biological replicates). FC, fold change. (B) Biocarta pathway enrichment analysis of SGI1027-induced genes in RCC10 cells. (C) RT-qPCR analysis of TNFSF10 , TNFRSF10A , TNFRSF10B , and TNFRSF10D mRNA levels in RCC10 cells treated with vehicle or SGI1027 for 2 days ( n = 3 biological replicates). (D and E) Immunoblot analysis of TRAIL, DR4, DR5, DcR2, pro-caspase-10, and cleaved caspase-10 (C-caspase-10) proteins in isogenic RCC10 cells treated with vehicle, SGI1027 (D and E, n = 2 biological replicates), MS1129 (E, n = 2 biological replicates), or decitabine (E, n = 2 biological replicates) for 2 or 7 days. (F) Global m5C levels in RCC10 cells treated with vehicle or SGI1027 for 2 days by ELISA assay ( n = 3 biological replicates). (G–J) MeDIP-qPCR assay in RCC10 cells treated with vehicle or SGI1027 for 2 days ( n = 3 biological replicates). (K–M) DNMT1, DNMT3A, and DNMT3B ChIP-qPCR assay in RCC10 cells ( n = 3 biological replicates). (N) Scheme of dDNMT-activated apoptotic pathway. Data represent mean ± SEM. p value was determined by bioinformatics with edgeR (A) or gene set enrichment analysis (B), unpaired 2-tailed Student’s t test (C and F), two-way ANOVA with Tukey’s test (G–I), and one-way ANOVA with Dunnett’s test (K–M). See also and .

    Article Snippet: anti-DR4 antibody , Proteintech , 24063-1-AP, RRID: AB_2879421.

    Techniques: Quantitative RT-PCR, Western Blot, Enzyme-linked Immunosorbent Assay, Methylated DNA Immunoprecipitation, ChIP-qPCR

    dDNMT specifically kills patient-derived VHL -deficient ccRCC in mice (A) Tumor growth curves of VHL -deficient UTSW-PDX206, UTSW-PDX258, UTSW-PDX490, and UTSW-PDX26 in mice treated with vehicle (Veh) or SGI1027 for 10 days. (B) Tumor growth curves of VHL -WT UTSW-PDX416 and UTSW-PDX143 in mice treated with vehicle or SGI1027 for 10 days. (C) Kaplan-Meier survival curve of UTSW-PDX490-bearing mice ( n = 10 biological replicates). (D and E) Global m5C levels in UTSW-PDX206 (D) or UTSW-PDX258 (E) tumors harvested from mice after treatments by ELISA assay ( n = 5 biological replicates). (F) Immunoblot analysis of DNMT1, DNMT3A, DNMT3B, TRAIL, DR4, DR5, procaspase-10, C-caspase-3, and C-caspase-7 proteins in UTSW-PDX258 tumors harvested from mice after treatments ( n = 5 biological replicates). (G) Representative C-caspase-3 IHC in UTSW-PDX258 tumors. Scale bar, 100 μm. (H) Quantification of C-caspase-3-positive cells in (G) ( n = 5 biological replicates). (I) Immunoblot analysis of DNMT1, DNMT3A, DNMT3B, TRAIL, DR4, DR5, C-caspase-3, and VHL proteins in UTSW-PDX416 tumors harvested from mice after treatments ( n = 5 biological replicates). (J) Immunoblot analysis of DNMT1, DNMT3A, DNMT3B, and procaspase-10 proteins in UTSW-PDX206, UTSW-PDX258, and UTSW-PDX26 tumors ( n = 4–5 biological replicates). Data represent mean ± SEM. p value was determined by two-way ANOVA with Tukey’s test (A), log rank test (C), and unpaired 2-tailed Student’s t test (D, E, and H). See also and ; .

    Journal: Cell Reports Medicine

    Article Title: HIF-activated priming of TRAIL-induced cell death determines epigenetic vulnerability in kidney cancer

    doi: 10.1016/j.xcrm.2026.102630

    Figure Lengend Snippet: dDNMT specifically kills patient-derived VHL -deficient ccRCC in mice (A) Tumor growth curves of VHL -deficient UTSW-PDX206, UTSW-PDX258, UTSW-PDX490, and UTSW-PDX26 in mice treated with vehicle (Veh) or SGI1027 for 10 days. (B) Tumor growth curves of VHL -WT UTSW-PDX416 and UTSW-PDX143 in mice treated with vehicle or SGI1027 for 10 days. (C) Kaplan-Meier survival curve of UTSW-PDX490-bearing mice ( n = 10 biological replicates). (D and E) Global m5C levels in UTSW-PDX206 (D) or UTSW-PDX258 (E) tumors harvested from mice after treatments by ELISA assay ( n = 5 biological replicates). (F) Immunoblot analysis of DNMT1, DNMT3A, DNMT3B, TRAIL, DR4, DR5, procaspase-10, C-caspase-3, and C-caspase-7 proteins in UTSW-PDX258 tumors harvested from mice after treatments ( n = 5 biological replicates). (G) Representative C-caspase-3 IHC in UTSW-PDX258 tumors. Scale bar, 100 μm. (H) Quantification of C-caspase-3-positive cells in (G) ( n = 5 biological replicates). (I) Immunoblot analysis of DNMT1, DNMT3A, DNMT3B, TRAIL, DR4, DR5, C-caspase-3, and VHL proteins in UTSW-PDX416 tumors harvested from mice after treatments ( n = 5 biological replicates). (J) Immunoblot analysis of DNMT1, DNMT3A, DNMT3B, and procaspase-10 proteins in UTSW-PDX206, UTSW-PDX258, and UTSW-PDX26 tumors ( n = 4–5 biological replicates). Data represent mean ± SEM. p value was determined by two-way ANOVA with Tukey’s test (A), log rank test (C), and unpaired 2-tailed Student’s t test (D, E, and H). See also and ; .

    Article Snippet: anti-DR4 antibody , Proteintech , 24063-1-AP, RRID: AB_2879421.

    Techniques: Derivative Assay, Enzyme-linked Immunosorbent Assay, Western Blot